Research Article

Effect of Moringa oleifera, Bacillus amyloliquefaciens, and Their Combination on Growth Performance, Digestive Enzymes, Immunity, and Microbiota in Nile Tilapia (Oreochromis niloticus)

Table 3

The growth performances of Nile tilapia fed test diets for 56 days.

TreatmentsCONMOP values
CONBaCONBaMOBaMO × Ba

FBW (g)164.27 ± 0.59167.38 ± 1.62151.95 ± 1.52157.46 ± 1.38<0.001 (b/a)0.012 (x/y)0.396
WGR (%)368.78 ± 1.74377.61 ± 4.70333.68 ± 4.27349.42 ± 3.99<0.001 (b/a)0.013 (x/y)0.395
SGR (g/day %)2.76 ± 0.012.79 ± 0.022.62 ± 0.022.68 ± 0.02<0.001 (b/a)0.009 (x/y)0.391
FI (g/fish/day)2.40 ± 0.152.46 ± 0.062.08 ± 0.062.15 ± 0.060.045 (b/a)0.5490.904
FCR1.06 ± 0.111.10 ± 0.030.95 ± 0.040.96 ± 0.020.007 (b/a)0.4560.706
PER (%)2.77 ± 0.272.69 ± 0.063.20 ± 0.113.14 ± 0.050.001 (a/b)0.4680.883
SR (%)91.67 ± 0.8392.50 ± 3.8298.13 ± 0.0093.33 ± 4.410.4260.6610.536

Notes: MO, M. oleifera supplementation; Ba, B. amyloliquefaciens supplementation; MO × Ba, interaction effect. Values are means and standard error of the mean (SEM). ns, no significant, . Different letters indicate significant differences between the two woody types and probiotic types, such as (a/b) in parentheses corresponds to no MO addition, MO addition, and the value of a is less than b. The other set of letters (x/y) corresponds to no B. amyloliquefaciens addition and B. amyloliquefaciens addition, and the value of x is less than the value of y. IBW (g), initial body weight; FBW (g), final body weight; Survival rate (SR, %) = 100 × final number of the fish/40; Weight gain rate (WGR, %) = (FBW − IBW)/IBW; Specific growth rate (SGR, %/day) = 100 × (lnFBW − lnIBW)/days; Protein efficiency ratio (PER, %) = weight gain (g)/protein intake (g); Feed conversion ratio (FCR) = feed intake (dry weight, g)/weight gain (g); Feeding intake (FI, g/fish/day) = feed intake (dry weight, g) × 100/((IBW + FBW)/2 × days).