Potential Targets and Signaling Mechanisms of Cinnamaldehyde Enhancing Intestinal Function and Nutritional Regulation in Fat Greenling (Hexagrammos otakii)

Figure 1

The flowchart of the experimental culture trial and exercise workflow diagram of in the ameliorate of intestinal inflammation disease (IIF). Stage 1—screening and selection of targets for cinnamaldehyde; Stage 2— adding different concentrations of cinnamaldehyde to feed the H. otakii; Stage 3—experimental validation against selected targets. All tests were performed in laboratory conditions. The relevant targets of cinnamaldehyde and intestinal inflammation were first searched through drug and disease-related databases, followed by Wayne plots, enrichment analysis, and PPI network analysis to establish the targets at the intersection of cinnamaldehyde and intestinal inflammation as well as their interrelationships. Subsequently, we conducted in vivo experiments by supplementation of cinnamaldehyde to the diets of H. otakii, and finally validated the predicted targets by observing the histological morphology and structure and quantifying the inflammatory genes.