Potential Targets and Signaling Mechanisms of Cinnamaldehyde Enhancing Intestinal Function and Nutritional Regulation in Fat Greenling (Hexagrammos otakii)

Table 2

Sequences of primers used in qRT-PCR.

Gene	Primer sequence (5^′–3^′)
C5 - F	GCGACTCCTCTGGTTGTATAG
C5 - R	GAACAGGAAGTGAGCGTATGT
C5aR1 - F	GAACACGAGGCCGTAGAAG
C5aR1 - R	GGAGACTTCAATTCCACCAATTAC
TLR4 - F	GGAATGTTGCTCAGTTGTCTCT
TLR4 - R	CAGGCGAGTCAGATACTTCAGA
P65 - F	GACTGCAAACACGGCTACTA
P65 - R	GGCCTCATTCACATCCTTCTT
PTGS2 - F	CATTGAAGGTCGGAGGACTATC
PTGS2 - R	CGTCAGCAACATCTCCTTCT
MyD88 - F	TAGTCGCATATGGTGAGGAAAC
MyD88 - R	GTCCCGGAGCTGAAAGTAAA
TNF-α - F	CTTCTACCAGTACGCACATCC
TNF-α - R	AACACTCAGACAGCCATACAC
IL-6 - F	GTCTGTATCTGGCCGTGATATG
IL-6 - R	ATGACCGTTACCTGGAGTTTG
β - Actin	CTGGTCTGGATTGGCTGTGA
β - Actin	GGAAGGAAGGCTGGAAGAGG

Abbreviations. C5, C5 complement; C5aR1, C5a complement receptor 1; TLR4, toll-like receptor 4; p65, transcription factor p65; PTGS2, prostaglandin G/H synthase 2; MyD88, myeloid differentiation primary response gene 88; TNF-α, tumor necrosis factor alpha; IL-6, interleukin 6.