Investigation of the Protective Effect of Probiotic Lactobacillus plantarum Ep-M17 on the Hepatopancreas of Penaeus vannamei

Figure 1

Hepatopancreas histology staining was performed using hematoxylin-eosin. Shrimp were divided into two groups and fed different diets: the CDG group fed basal diet, while the RDG group received a basal diet containing L. plantarum Ep-M17. Samples were collected at three different time points: the second (2 W) and fourth weeks (4 W) of feeding, and the 48 hr after the V. parahaemolyticus E1 attack (cp-2d). In the stained samples, R cells were observed to contain lipid vacuoles of varying sizes. F cells exhibited abundant ribosomes and well-developed endoplasmic reticulum. B cells typicall displayed a single large secretory vesicle. The green double arrows indicate the surface cell epithelium, while yellow triangles mark the cell interstices. Bar: 100 μm. (a) Feeding with basal diet for the second week (2 W); (b) feeding with basal diet for the fourth week (4 W); (c) control group at 48 hours after V. parahaemolyticus E1 challenge (cp-2d); (d) feeding with diet supplemented with L. plantarum Ep-M17 for the second week (2 W); (e) feeding with diet supplemented with L. plantarum Ep-M17 for the fourth week (4 W); and (f) experimental group at 48 hours after V. parahaemolyticus E1 challenge (cp-2d).