Betaine Alleviates High-Fat Diet Induced Excessive Lipid Deposition in Gibel Carp Hepatopancreas and L8824 Cells by Enhancing VLDL Secretion through HNF4α/MTTP Pathway

Table 1

Information of primers used in this study.

Primers^1	Sequence 5′−3′ ^2	Purpose	Efficiency (%)
H-MTTP forward	TTATGAGCAGTAGTCCGTCTT	Hepatopancreas qRT-PCR	104.52
H-MTTP reverse	GACCCAGTCTTTGAGAACCT
H-HNF4α forward	ATCCCAGCATTCTGTGACCTT	Hepatopancreas qRT-PCR	98.69
H-HNF4α reverse	TGGAAGGGAAGCACTAACTCAT
H-ApoB forward	AGCAGCAACTTCTACAACT	Hepatopancreas qRT-PCR	103.14
H-ApoB reverse	TTATGGATAGGAGGTCAACAG
H-EF-1α forward	CGCCAGTGTTGCCTTCGT	Hepatopancreas qRT-PCR	99.86
H-EF-1α reverse	CGCTCAATCTTCCATCCCTT
C-MTTP forward	TCAGGATACCACTATGGAGTTCAC	L8824 cell qRT-PCR	98.61
C-MTTP reverse	TACAGCGGCGTTCACACTTG
C-HNF4α forward	TGGTGCTGGTGGAATGG	L8824 cell qRT-PCR	99.08
C-HNF4α reverse	ATCTTGGAAGGGAAGGACTAA
C-ApoB forward	CGTATTGCTGCCTATCTCATT	L8824 cell qRT-PCR	100.96
C-ApoB reverse	CTTAGTTTCTGGTGCTGTGGA
C-β-actin forward	AAGTATCCTATTGAGCACGGTATTG	L8824 cell qRT-PCR	100.44
C-β-actin reverse	TCAGGGGAGCCTCTGTAAGC
L-MTTP forward	TAGGTACCCAAGATAAGCAGCATCAACACCA	Dual-luciferase assay	—
L-MTTP reverse	CCAAGCTTGGTTGGCTCGCTGAGAGCTGTG
L-ApoB forward	TAGGTACCCTAATCACTGTGTTTTAT	Dual-luciferase assay	—
L-ApoB reverse	CCAAGCTTCGTTATGGGCACTGAAACTTC
L-HNF4a forward	CCGGAATTCATGCGTTTGTCCAAACCAC	Dual-luciferase assay	—
L-HNF4a reverse	CCGCTCGAGTCAGATGGCCTCTTGTTTGG

¹The prefix of the primer name indicates the purpose of the primer. H, Hepatopancreas; C, L8824 cell; L, Dual-luciferase assay. ²The underscored bases represent the recognition sequence of a restriction endonuclease.