A Unique Combination of Mn²⁺ and Aluminum Adjuvant Acted the Synergistic Effect

Figure 4

Evaluation of humoral immune response. (a) The detection of antibody levels in mice. The sera in mice were separated and the level of anti-IsdB3 antibodies was analyzed by ELISA fourteen days after the last immunization. (b) The opsonization of the antibody was evaluated by opsonophagocytosis assay. After the sera from immunized mice were incubated with S. aureus strain Newman at 37°C for 2 h, this mixture was used to treat peritoneal macrophages from mice at 37°C for 4 h and then the extracellular S. aureus Newman strains were removed by gentamicin of 100 μg/mL. Finally, the number (CFU) of bacteria phagocytosed from macrophages was determined in each group. The data were shown as the mean ± SD (n = 3). Significant differences were indicated by , , and .

(a)

(b)