Knockout of Noxa with CRISPR/Cas9 Increases Host Resistance to Influenza Virus Infection

Figure 1

CRISPR/Cas9-mediated Noxa-knockout cells were generated and identified. (a) Schematic of gRNAs targeting Noxa. Sanger sequence showed a 392 bp inset in exon2. (b) Noxa mRNA expression was detected via qPCR analysis. The location of qPCR primers is shown. (c) Western blotting results showing that there is no expression in mutant A549 cells with Noxa gRNAs, comparing with wild-type cells.

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