Research Article

Knockout of Noxa with CRISPR/Cas9 Increases Host Resistance to Influenza Virus Infection

Figure 3

Loss of Noxa increases LC3 accumulation and reduces p62 expression. (a) Immunoblot analysis of LC3 and p62 expression in wild-type and Noxa-deficient A549 cells. β-Actin was used as the loading control. (b) Wild-type and Noxa-deficient A549 cells were transfected with a plasmid encoding GFP-LC3 for 24 h and then fixed and stained with DAPI (blue). Scale bars, 10 μm. (c) Immunoblot analysis of LC3 and p62 expression in wild-type and Noxa-deficient A549 cells 8 h and 24 h postinfection. β-Actin was used as the loading control.
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