Knockout of Noxa with CRISPR/Cas9 Increases Host Resistance to Influenza Virus Infection

Figure 4

Noxa-deficient results in the autophagic degradation of influenza virus particles. (a) Wild-type and Noxa-deficient A549 cells were infected with the A/WSN/33 (H1N1) virus for 8 h. Representative images of immunofluorescence staining in cells with antibodies against Lamp2 (green) and LC3 (red). Blue: positive staining of DAPI. Scale bars, 10 μm. (b) Wild-type and Noxa-deficient A549 cells were infected with the A/WSN/33 (H1N1) virus for 8 h. Representative images of immunofluorescence staining in cells with antibodies against NP (green) and LC3 (red). Blue: positive staining of DAPI. Scale bars, 10 μm.

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