G₁ Cell Cycle Arrest Is Induced by the Fourth Extracellular Loop of Meningococcal PorA in Epithelial and Endothelial Cells

Figure 7

Localization of p-CDK4 in infected HBMECs. HBMECs were left uninfected or were infected with wild-type N. meningitidis MC58 or its isogenic derivative MC58 ΔPorA-Loop4 at MOI 500 for 8 h, 37°C, and 5% CO₂. Cells were fixed with 4% paraformaldehyde and stained with-anti-p-CDK4 (1 : 500) overnight, followed by secondary Alexa antibody (Alexa Fluor 680, red) overnight. Primary antibody was omitted in control images. Merged images also show DAPI staining (blue). Coverslips were mounted using ProLong Gold antifade mounting reagent. . Images representative of at least 3 independent experiments.