Commonality of Virulence-Promoting Function in Rhodococcus equi Virulence Associated Proteins (Vaps)

Figure 2

Vap-induced lysosomal swelling is not unique to VapA. (a). NRK cells were incubated without (Ctrl) or with 40 μg/ml recombinant Vaps (denoted by alphabetic character) for 20 h. Cells were fixed and the lysosomes immunolabelled with an anti-LGP120 monoclonal antibody, followed by an Alexa Fluor 555-labelled secondary antibody (red). Cell nuclei were stained with DAPI (blue). Scale bars 10 μm. (b). The size of lysosomes in the images was measured using automatic thresholding and quantification with ImageJ. Data are from 30 images in total from three separate experiments (10 images per experiment). Data are (). vs. control.

(a)

(b)