Commonality of Virulence-Promoting Function in Rhodococcus equi Virulence Associated Proteins (Vaps)

Figure 3

VapA, VapB, VapK, and VapN cause accumulation of LC3 in NRK cells. (a) NRK cells were incubated without (Ctrl) or with 40 μg/ml recombinant Vaps (denoted by alphabetic character) for 20 h. Cells were fixed and immunolabelled for LGP110 (green) and LC3 (red) before confocal imaging. Scale bars 10 μm. (b) NRK cells were incubated with protein as in (a) before lysing, and equal amounts of protein were immunoblotted for ɑ-tubulin or total LC3 (I and II).

(a)

(b)