Commonality of Virulence-Promoting Function in Rhodococcus equi Virulence Associated Proteins (Vaps)

Figure 4

VapA, VapB, VapK, and VapN can restore virulence in ΔvapA R. equi. J774A.1 cells were infected with R. equi strains 103+ (blue), ΔvapA (red), or 103- (green) without or in the presence of recombinant Vaps (5 μg/ml). Cells were lysed at 8 or 24 h, and viable cfu was determined. Data shown are relative change in cfu at 24 h compared to the postinfection (PI). Data are (). Statistics show Dunnett’s multiple comparison tests for each R. equi strain compared to the no-protein sample. , . VapB and VapN were also used at 1 μg/ml as denoted by B1 and N1, respectively.