Commonality of Virulence-Promoting Function in Rhodococcus equi Virulence Associated Proteins (Vaps)

Figure 5

Vaps A, B, K, and N vary in their ability to promote lysosome dysfunction. NRK cells were incubated with or without varying concentrations of Vaps A, B, K, and N for 24 h. Cells were fixed and the lysosomes immunolabelled with an anti-LGP120 monoclonal antibody, followed by an Alexa Fluor 555-labelled secondary antibody. The lysosome sizes of images were quantified using automatic thresholding and quantification with ImageJ. Data are from the analyses of 10 images per condition (). Control lysosomal size is indicated by the dashed horizontal line.