Commonality of Virulence-Promoting Function in Rhodococcus equi Virulence Associated Proteins (Vaps)

Figure 8

Vaps A, B, K, and N reduce lysosomal proteolytic activity resulting in swollen compartments containing undigested material. (a) Representative flow cytometry overlay histogram of DQ-BSA fluorescence in cells treated with or without Vaps and bafilomycin-A₁. 5,000 cells were counted for each condition. (b) Quantification of DQ-BSA fluorescence in cells as shown in (a). Data are mean median fluorescence intensity (). , , . (c) Holotomography of lysosomal glycoprotein-120-GFP (LGP120-GFP) Normal Rat Kidney (NRK) cell treated with VapA. Images were acquired every 10 min, and images shown represent the 0 h and 24 h images posttreatment. Refractive index (R.I.) tomographs (left panels), GFP fluorescence (middle panels), and refractive index rendering into low-density (blue) and high-density (red) regions (right panels). (d) Quantification of holotomography data (for 60 h) from the cell shown in (c). Data shown indicate the cell volume, dry mass, and overall R.I. of the cell.

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