Hypoxia-Inducible Factor-1α Regulates High Phosphate-Induced Vascular Calcification via Type III Sodium-Dependent Phosphate Cotransporter 1

Figure 1

Changes in HIF-1α protein expression. HASMCs were cultured in normal DMEM media and transfected with HIF-1α siRNA (a) or an overexpression plasmid (b). The expression levels of the HIF-1α protein were examined by western blot analysis at 24 h following transfection. The levels were normalized to those of tubulin. The data shown are indicative of mean ± SD. ^∗p < 0.05 vs. control; vs. control. HIF-1α, hypoxia-inducible factor 1 alpha; HASMCs, human aortic smooth muscle cells; DMEM, Dulbecco’s modified Eagle’s medium; siRNA, small interfering RNA.

(a)

(b)