Hypoxia-Inducible Factor-1α Regulates High Phosphate-Induced Vascular Calcification via Type III Sodium-Dependent Phosphate Cotransporter 1

Figure 6

Changes in calcium deposition. (a) Reddish brown coloration by Alizarin red staining indicates calcium deposits in cultured HASMCs (magnification ×200). (b) The quantitative evaluation of calcium deposition in cultured HASMCs was examined by the o-cresolphthalein complexone method. The data shown are indicative of mean ± SD. vs. CNT; ^# vs. HP; ^&& vs. HPOE. CNT, HASMCs cultured under normal conditions with 0.9 mM Pi; HP, HASMCs cultured with 2.5 mM Pi; HPSI, HIF-1α siRNA-transfected HASMCs cultured with 2.5 mM Pi; HPOE, HIF-1α overexpression plasmid-transfected HASMCs cultured with 2.5 mM Pi; HPOEPFA, HIF-1α overexpression plasmid-transfected HASMCs cultured with 2.5 mM Pi and 0.5 mM PFA. HASMCs, human aortic smooth muscle cells; SD, standard deviation; CNT, control; HP, high Pi; HPOE, overexpression and high Pi; Pi, inorganic phosphorous; HPSI, siRNA and high Pi; HIF-1α, hypoxia-inducible factor 1 alpha; siRNA, small interfering RNA; HPOEPFA, overexpression high Pi and phosphonoformic acid group; PFA, phosphonoformic acid. Scale bar = 20 μm.

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