Characterization of Rhizobia Isolated from Tigray Soil and Assessment of Their Effect on Germination and Seedling Vigor of Wheat and Field Pea

Table 8

Effect of rhizobia on wheat seed germination and 7-day-old seedling growth parameters.

Isolates	GP	SSL (cm)	SRL (cm)	SVI
SH1	97.0 ± 3.00^def	4.96 ± 0.55^lmo	6.52 ± 0.49^hij	1113 ± 3.5^o
SH2	99.0 ± 1.00^abc	6.43 ± 0.61^ghij	6.52 ± 0.57^hij	1283 ± 2.0^i
SH3	98.0 ± 0.00^cd	4.79 ± 0.31^mno	4.90 ± 0.44^lmo	951 ± 1.5^r
SH4	96.3 ± 1.15^ef	6.26 ± 1.28^ghijk	7.25 ± 0.62^fgh	1301 ± 0.9^i
SH5	95.8 ± 0.57^f	5.86 ± 0.41^hijkl	6.14 ± 0.52^ijk	1148 ± 4.5^mn
KO1	99.7 ± 0.57^ab	11.60 ± 0.51^a	8.57 ± 0.48^de	2012 ± 1.6^b
KO2	99.3 ± 0.57^abc	7.85 ± 0.53^de	9.28 ± 0.65^cd	1702 ± 7.2^f
KO3	100.0 ± 0.00^a	6.06 ± 0.54^ghijk	7.83 ± 0.55^efg	1389 ± 9.5^h
KO4	100.0 ± 0.00^a	9.86 ± 0.64^b	6.96 ± 0.71^ghi	1683 ± 11.4^f
KO5	99.3 ± 0.57^abc	7.56 ± 0.52^def	7.24 ± 0.64^fgh	1471 ± 11.0^g
KO6	99.0 ± 1.00^abc	4.33 ± 0.39^o	4.42 ± 0.52^no	866 ± 22.2^t
KO7	98.3 ± 1.52^bcd	5.81 ± 0.58^ijkl	5.73 ± 0.56^jkl	1136 ± 10.3^no
KO8	98.0 ± 1.00^cd	5.90 ± 0.49^hijk	5.14 ± 0.42^lmo	1082 ± 17.1^p
TS1	99.0 ± 1.00^abc	5.32 ± 0.56^klmn	5.46 ± 0.44^klm	1069 ± 23.7^p
TS2	98.0 ± 0.00^cd	6.29 ± 0.53^ghij	5.77 ± 0.41^jkl	1183 ± 12.1^kl
TS3	99.3 ± 0.57^abc	5.84 ± 0.49^hijkl	6.77 ± 0.55^hi	1253 ± 43.9^j
ME1	98.3 ± 0.57^bcd	4.82 ± 0.54^mno	5.25 ± 0.49^klmn	991 ± 15.6^q
ME2	99.7 ± 0.57^ab	6.97 ± 0.58^efg	10.13 ± 0.37^c	1704 ± 6.7^f
ME3	100.0 ± 0.00^a	8.39 ± 0.23^cd	13.90 ± 0.56^a	2230 ± 12.1^a
ME4	98.3 ± 0.57^bcd	5.82 ± 0.34^ijkl	6.42 ± 0.41^hij	1204 ± 28.9^k
ME5	97.3 ± 1.52^de	5.55 ± 0.67^jklm	6.14 ± 0.74^ijk	1138 ± 45.3^no
TA1	99.7 ± 0.57^ab	8.04 ± 0.35^cd	11.37 ± 0.61^b	1935 ± 6.8^d
TA2	99.7 ± 0.57^ab	8.81 ± 0.18^c	11.29 ± 0.65^b	2004 ± 7.8^bc
TA5	100.0 ± 0.00^a	7.48 ± 0.74^def	9.98 ± 0.52^c	1747 ± 12.5^e
TA7	99.0 ± 1.00^abc	11.70 ± 0.71^a	8.32 ± 0.33^e	1983 ± 3.4^c
TA8	99.7 ± 0.57^ab	5.32 ± 0.65^klmn	6.40 ± 0.68^hij	1170 ± 10.3^lm
TA9	99.0 ± 1.00^abc	6.69 ± 0.38^fghi	8.06 ± 0.67^ef	1461 ± 10.5^g
TA10	99.7 ± 0.57^ab	6.76 ± 0.53^fgh	8.11 ± 0.58^ef	1483 ± 8.2^g
NC	96.0 ± 0.00^ef	4.71 ± 0.59^mno	4.27 ± 0.63^o	862 ± 2.6^t
PC	99.3 ± 0.57^abc	4.61 ± 0.56^no	4.59 ± 0.62^mno	915 ± 3.0^s
LSD	1.65	0.94	0.92	26.25
SEm (±)	0.18	0.1	0.1	2.93
F-probability	<0.001	<0.001	<0.001	<0.001
CV (%)	1.02	8.6	7.75	1.16

Germination and shoot/root data are means of 3 replications and each replicate of the shoot/root length was an average of 10 wheat seedlings per petri-plates harvested after 7 days. Means in the same column that are followed by different letters are significantly different at using Fisher’s LSD test; LSD: least significant difference; CV: coefficient of variation (%); SEm: standard error of mean NC: negative control; PC: positive control; GP: germination percentage; SSL: seedling shoot length; SRL: seedling root length; SVI: seedling vigor index.