Facile Conversion and Optimization of Structured Illumination Image Reconstruction Code into the GPU Environment

Figure 11

The combination of improved hardware and code produces an image stack identical to the original Hessian-SIM denoising algorithm, but at 4- to 500-fold faster rates. (a–c) Frame 50 from the image stack produced using the (a) original algorithm and (b, c) the improved algorithms, executed on superior cost-effective computers as shown in (d). Line profile analysis was performed using the Plot Profile function in Fiji [31]. The resulting data were exported into GraphPad Prism (v8.43) and displayed on the same axes for direct comparison.

(a)  sec

(b)  sec

(c)  sec

(d)