Research Article
RuX: A Novel, Flexible, and Sensitive Mifepristone-Induced Transcriptional Regulation System
Figure 10
Structures of regulator and responder plasmids used for creating the Cre-RuX system. Regulator plasmids (0030.6, 0030.6M10, 0030.6M10t, 0030.6M11, 0030.6M11t, and 0030.16) express chimeric molecules composed of a fusion of different variations of a humanized Cre sequence and the GRLBD CS1/CD 504 fragment. The 0030.17 responder plasmid consists of a CMV promoter-driven and floxed EGFP, polyadenylated by a strong artificial poly(A) element containing polyadenylation signals from the bovine globin gene and SV40 (“3x poly(A),” green box). This strong terminator completely blocks readthrough into the downstream Gluc. 0030.14 is a benchmarking plasmid containing the Cre-ERT2 system in the same structural setup as in the Cre-RuX constructs.