RuX: A Novel, Flexible, and Sensitive Mifepristone-Induced Transcriptional Regulation System

Figure 3

The structure of plasmid constructs CA97.2 (the base version of Universal Fusion Backbone) and the RuX activator plasmid family (CA97.62, CA97.63, CA97.64, CA97.65, CA97.66, CA97.67, CA97.75, CA97.76, CA97.86, and CA97.87) based on this construct. The base construct consists of three promoters and three linkers in-frame. Unique restriction sites (not shown) allow excising any two promoters and cloning cDNA of interest in between the linkers. Different Gal4 DNA-binding domain mutants (GAL DBD, blue box) were used in combination with different GR^LBD CS1/CD mutants (BR LBD, red box). Flexible linkers (“3x GGGS,” orange box) and a rigid linker (“8x EAAAK,” yellow box) are shown. The relative sizes of the elements are not proportional. Plasmid backbones are not shown.