International Journal of Cell Biology

Research Article

RuX: A Novel, Flexible, and Sensitive Mifepristone-Induced Transcriptional Regulation System

Table 1

List of plasmid constructs created and used in the study (GS stands for GeneSwitch).
Plasmid nameFunctionRemark
CA97.2Base constructCloning backbone
CA97.67RuX regulatorWild-type Gal4 DBD × GRLBD 540
CA97.62RuX regulatorGal4 DBD Mut1 × GRLBD 540
CA97.63RuX regulatorGal4 DBD Mut2 × GRLBD 540
CA97.64RuX regulatorGal4 DBD Mut3 × GRLBD 540
CA97.65RuX regulatorGal4 DBD Mut4 × GRLBD 540
CA97.66RuX regulatorGal4 DBD Mut5 × GRLBD 540
CA97.75RuX regulatorGal4 DBD Mut6 × GRLBD 540
CA97.76RuX regulatorGal4 DBD Mut7 × GRLBD 540
CA97.86RuX regulatorGal4 DBD Mut7 × GRLBD 524
CA97.87RuX regulatorGal4 DBD Mut7 × GRLBD 504
E000.29RuX responderTesting RuX regulators, Gluc reporter
ST04.2RuX responderTesting RuX regulators, Nluc-P reporter
ST04.4GS responderBenchmarking GS, Gluc reporter
ST04.5GS responderBenchmarking GS, Nluc-P reporter
0030.6M10RuX-Cre regulatorCre R119C × GRLBD 504
0030.6M11RuX-Cre regulatorR119V × GRLBD 504
0030.6M10tRuX-Cre regulatorCre R119C d(1-18) × GRLBD 504
0030.6M11tRuX-Cre inducerCre R119V d(1-18) × GRLBD 504
0030.6RuX-Cre regulatorhuCre × GRLBD 504
0030.16RuX-Cre regulatorhuCre V336A × GRLBD 504
0030.14Cre-ERT2 regulatorCre-ERT2 adapted for testing against RuX-Cre
0030.17Cre responderEGFP expression switches to Gluc upon Cre
ST00.1Internal controlConstitutive SEAP-expressing plasmid