RuX: A Novel, Flexible, and Sensitive Mifepristone-Induced Transcriptional Regulation System

<div>Performance benchmarking of Cre-RuX mutants against Cre-ERT2 HeLa cells, 48 h posttransfection, compared to baseline right after transfection. Induction rates are shown as relative Gaussia measurements normalized for transfection rates. The NLS-mutant 0030.6M011 has improved induction rates at any amount of plasmid transfected, compared to the best achievable rates with the Cre-ERT2 construct 0030.14. The base construct, 0030.6, is functional but outperformed by the improved versions 0030.6M10t and 0030.6M11t. The V336A mutant 0030.16 has high induction rates only at high transfection concentrations.</div>

International Journal of Cell Biology

tab3

Table 3

Table 3: RuX: A Novel, Flexible, and Sensitive Mifepristone-Induced Transcriptional Regulation System 