Comparative Transcriptomic Analyses of a Vero Cell Line in Suspension versus Adherent Culture Conditions

Table 4

Proposed strategies for Vero cell adaptation to suspension improvement.

Phenotype observed	Proposed improvement
Long doubling time	(i) Gene editing: targeted knock-out of FYN gene; (ii) Gene editing: enhance upregulation of RHOU and ESR1; (iii) Medium formulation: upregulation of glycine, threonine, and serine intake; (iv) Medium formulation: reduce folate intake
Low cell density	(i) Gene editing: enhance upregulation of adherens junction-related genes; (ii) Gene editing: enhance upregulation of MYC and E2F; (iii) Medium formulation: upregulation aspartate intake; (iv) Medium formulation: upregulate mitochondrial 1-carbon metabolism
Adhesion	(i) Gene editing: targeted knock-out of CDH18