Acute Genetic Damage Induced by Ethanol and Corticosterone Seems to Modulate Hippocampal Astrocyte Signaling

Figure 1

Primary DNA damage induced by EtOH and/or CTS in cultured astrocytes. (a) DIC and confocal images evidencing the DNA-damaged sites recognized as γH2AX (red) foci in DAPI-stained nuclei (blue) as seen in confocal images on the right side of the panel. Left and central images depict γH2AX and DAPI signals on DIC images, respectively. Calibration bar: 5 μm. (b) Box plots show the number of γH2AX foci per astrocyte in each experimental condition. Boxes enclose the data between the 25th and 75th percentiles. The median (50th percentile) is indicated by the cross line within each box. Differences between 100 astrocytes per analyzed variable and group were examined using the Kruskal-Wallis test with Dunn test for multiple comparisons. ; ^ns (not significant). DIC: differential interference contrast; DAPI: 2-(4-amidinophenyl)-1H-indole-6-carboxamidine; CM: culture media; DMSO: dimethyl sulfoxide; CTS: corticosterone; EtOH: ethanol; EtOH+CTS: simultaneous EtOH and CTS treatment.

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