Acute Genetic Damage Induced by Ethanol and Corticosterone Seems to Modulate Hippocampal Astrocyte Signaling

Figure 6

Distinctive appearance of astrocyte EVs related to control or exposure conditions. (a, b) SEM images depicting the shape and arrangement of EVs detected in controls or EtOH- and/or CTS-exposed astrocytes. Colored asterisks point to different EVs: red: round; blue: elongated; yellow: donut-shaped; green: drumstick-shaped; pink: cup-shaped; yellow arrowheads: rosette-shaped. (c) Diagram illustrating the EV forms observed on the astrocyte surfaces and their comparative distribution in the different experimental conditions (colored bars). (d) Ordered and disordered EV arrangements (red and green arrowheads, respectively). The phenotypic appearance of these EVs agrees, with minor differences, with the observation of Malenica et al. [23]. SEM: scanning electron microscopy; CM: culture media; DMSO: dimethyl sulfoxide; EtOH: ethanol; CTS: corticosterone; EtOH+CTS: EtOH and CTS coexposure; EVs: extracellular vesicles.

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