The Effect of Differentiation of Human Dental Pulp Stem Cells to Glial Cells on the Sensory Nerves of the Dental Pulp

Figure 2

Differentiation of hDPSCs into glial cells: (a) schematic diagram of the culture assay. hDPSCs cultured in β-ME medium for ∼1 day before further seeding in RA culture medium for 3 days, followed by culture in Forskolin, b-FGF, PDGFaa, and NRG-1 medium. Cells and cell supernatant were collected for further characterization; (b) immunofluorescent staining was performed on hDPSCs and SC-DPSCs for the typical Schwann cell markers MBP, p75NTR, and S-100. Scale bar: 20 μm. hDPSC, human dental pulp stem cell; PDGFaa, platelet-derived growth factor AA; SC, stem cell; (c) RT-PCR analysis of the expression of Schwann cell marker MBP, p75NTR, and S-100. b-FGF, basic fibroblast growth factor; PDGFaa, platelet-derived growth factor AA; NRG-1, neuregulin-1; MBP, myelin basic protein; p75NTR, p75 neurotrophin receptor; and β-ME, β-mercaptoethanol. .

(a)

(b)

(c)