Overexpression of FERM Domain Containing Kindlin 2 (FERMT2) in Fibroblasts Correlates with EMT and Immunosuppression in Gastric Cancer

Figure 2

FERMT2 is a biomarker of fibroblast infiltration in normal and pancancer tissues. (a) Uniform manifold approximation and projection (UMAP) plot showing FERMT2 expression in normal single-cell clusters (HPA database). (b) Heatmap showing gene expression in FERMT2-specific cluster. -axis shows sample types across 30 tissues, and -axis shows genes in selected clusters. The vertical line on the left indicates the confidence level of the gene assigned to the cluster. (c) The Gene Ontology (GO) tree shows the summary results of the GO term overexpression analysis. The most prominent terms are summarized as a single box with white text. Orange, red, and blue encode different GO term domains, respectively. (d) The heatmap of associations between FERMT2 level and fibroblast infiltration in pancancer was calculated by four algorithms on Tumor Immune Estimation Resource (TIMER) web tool. (e) FERMT2 expression in cancer single-cell clusters obtained from TISCH online tool. Spatial transcription sections showing the spatial expression of FERMT2, ACTA2, and DCN fibroblast marker in (f) cervical cancer (CESC), (g) prostate cancer (PRAD), (h) breast cancer (BRCA), (i) glioblastoma (GBM), (j) ovarian cancer (OV), and (k) kidney cell carcinoma (RCC). The dot color represents the expression level of the selected gene. Colocalization was highlighted by the red box. (l) Correlation between FERMT2 and tumor stroma scores assessed by the “Estimate” algorithm in pancancer. Spearman’s method was used.

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