Research Article

A Comparative Study on Phenotypic versus ITS-Based Molecular Identification of Dermatophytes Isolated in Dakar, Senegal

Table 1

Universal ITS primers and amplified regions [12].

Noun/pairUniversal primer sequence (5′ ⟶ 3′)Amplified regions

ITS1/ITS2ITS1: 5′-TCCGTAGGTGAACCTGCGG-3′3′end 18S, ITS1, and 5′ end 5, 8S
ITS2: 5′-GCTGCGTTCTTCATCGATGC-3′

ITS3/ITS4ITS3: 5′-GCATCGATGAAGAACGCAGC-3′3′ end 5, 8S, ITS2, and 5′ end 28S
ITS4: 5′-TCCTCCGCTTATTGATATGC-3′

ITS1/ITS4ITS1: 5′-TCCGTAGGTGAACCTGCGG-3′3′ end 18S, ITS1, 5, 8S, ITS2, and 5′ end 28S
ITS4: 5′-TCCTCCGCTTATTGATATGC-3′