Research Article
A Comparative Study on Phenotypic versus ITS-Based Molecular Identification of Dermatophytes Isolated in Dakar, Senegal
Table 1
Universal ITS primers and amplified regions [
12].
| Noun/pair | Universal primer sequence (5′ ⟶ 3′) | Amplified regions |
| ITS1/ITS2 | ITS1: 5′-TCCGTAGGTGAACCTGCGG-3′ | 3′end 18S, ITS1, and 5′ end 5, 8S | ITS2: 5′-GCTGCGTTCTTCATCGATGC-3′ |
| ITS3/ITS4 | ITS3: 5′-GCATCGATGAAGAACGCAGC-3′ | 3′ end 5, 8S, ITS2, and 5′ end 28S | ITS4: 5′-TCCTCCGCTTATTGATATGC-3′ |
| ITS1/ITS4 | ITS1: 5′-TCCGTAGGTGAACCTGCGG-3′ | 3′ end 18S, ITS1, 5, 8S, ITS2, and 5′ end 28S | ITS4: 5′-TCCTCCGCTTATTGATATGC-3′ |
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