Potential Antimicrobe Producer of Endophytic Bacteria from Yellow Root Plant (Arcangelisia flava (L.)) Originated from Enggano Island
Table 2
The antimicrobial assay of four selected endophytic isolates.
Sources
Isolate codes
Inhibition zone diameter (mm)
Pathogenic microbes
E. coli
S. aureus
P. aeruginosa
C. albicans
Pure isolates (first screening)
AKEBG21
2.70 ± 0.23
12.7 ± 1.19
0.00 ± 0.00
0.00 ± 0.00
AKEBG23
22.20 ± 1.20
23.8 ± 0.70
0.00 ± 0.00
0.00 ± 0.00
AKEBG25
12.70 ± 0.98
19.5 ± 0.45
0.00 ± 0.00
0.00 ± 0.00
AKEBG28
15.80 ± 1.75
16.7 ± 0.11
0.00 ± 0.00
0.00 ± 0.00
PC+
20.80 ± 0.22
22.40 ± 0.03
18.30 ± 017
25.08 ± 0.02
NC−
0.00 ± 0.00
0.00 ± 0.00
0.00 ± 0.00
0.00 ± 0.00
Pelleted cells (centrifuged cells)
AKEBG21
2.10 ± 0.56
1.40 ± 0.56
0.00 ± 0.00
0.00 ± 0.00
AKEBG23
4.00 ± 0.70
1.00 ± 0.00
0.00 ± 0.00
0.00 ± 0.00
AKEBG25
1.10 ± 0.07
0.50 ± 0.28
0.00 ± 0.00
0.00 ± 0.00
AKEBG28
3.10 ± 0.07
0.70 ± 0.28
0.00 ± 0.00
0.00 ± 0.00
PC+
23.60 ± 0.45
19.50 ± 0.54
18.34 ± 0.23
20.00 ± 0.01
NC−
0.00 ± 0.00
0.00 ± 0.00
0.00 ± 0.00
0.00 ± 0.00
Medium supernatant
AKEBG21
0.40 ± 0.14
0.15 ± 0.07
0.00 ± 0.00
0.00 ± 0.00
AKEBG23
3.00 ± 0.28
2.50 ± 0.42
0.00 ± 0.00
0.00 ± 0.00
AKEBG25
0.95 ± 0.07
1.65 ± 0.07
0.00 ± 0.00
0.00 ± 0.00
AKEBG28
2.40 ± 0.14
0.50 ± 0.28
0.00 ± 0.00
0.00 ± 0.00
PC+
23.60 ± 0.45
19.50 ± 0.54
18.34 ± 0.23
20.00 ± 0.02
NC−
0.00 ± 0.00
0.00 ± 0.00
0.00 ± 0.00
0.00 ± 0.00
Crude extract (ethyl acetate)
AKEBG21
19.10 ± 0.84
13.85 ± 0.49
3.15 ± 1.82
0.00 ± 0.00
AKEBG23
20.85 ± 0.07
23.15 ± 1.34
22.65 ± 0.21
0.00 ± 0.00
AKEBG25
19.80 ± 0.50
14.7 ± 0.28
11.65 ± 0.63
0.00 ± 0.00
AKEBG28
21.50 ± 0.42
14.6 ± 0.00
13.35 ± 0.63
0.00 ± 0.00
PC+
16.40 ± 0.28
19.5 ± 0.00
18.25 ± 0.63
16.0 ± 0.0
NC−
0.00 ± 0.00
0.00 ± 0.00
0.00 ± 0.00
0.00 ± 0.00
Positive control: streptomycin 1000 ppm for bacteria (S. aureus, E. coli, and P. aeruginosa; kanamycin 1000 ppm for C. albicans. Negative control: NC−: sterile TSB medium and NC−: ethyl acetate.
