Analysis of Amino Acid Changes in the Fusion Protein of Virulent Newcastle Disease Virus from Vaccinated Poultry in Nigerian Isolates
Table 10
Point mutation pattern along fusion protein of study isolates compared to reference LaSota strain KU665482.1.
ID virus isolate
Nucleotide at indicated position along the fusion gene
HRc
476
479
482
486
494
498
Codon
1426–1428
1435–1437
1444–1446
1456–1458
1480–1482
1492–1492
KU665482.1 LaSota.71.IR/2016†
AAT
AAT
GAC
AGA
AAA
ACA
OK491971 Avian orthoavulavirus 1 isolate KN 14‡
AAT
GAC (N479D)
GCA (E482A)
AAC (R486N)
AGA (K494R)
TCA (T498S)
OK491972 Avian orthoavulavirus 1 isolate KN 36‡
AAT
GAC (N479D)
GCA (E482A)
AAC (R486N)
AGA (K494R)
TCA (T498S)
OK491973 Avian orthoavulavirus 1 isolate KN 48‡
AAT
GAC (N479D)
GCA (E482A)
AAC (R486N)
AGA (K494R)
TCA (T498S)
OK491974 Avian orthoavulavirus 1 isolate KN 55‡
AAT
GAC (N479D)
GCA (E482A)
AAC (R486N)
AGA (K494R)
TCA (T498S)
OK491975 Avian orthoavulavirus 1 isolate KN 56‡
AAT
GAC (N479D)
GCA (E482A)
AAC (R486N)
AGA (K494R)
TCA (T498S)
OK491976 Avian orthoavulavirus 1 isolate KN 71‡
ACT (N476T)
GAC (N479D)
GCA (E482A)
AAC (R486N)
AGA (K494R)
TCA (T498S)
OK491977 Avian orthoavulavirus 1 isolate KN 75‡
AAT
GAC (N479D)
GCA (E482A)
AAC (R486N)
AGA (K494R)
TCA (T498S)
Variable positions along functional sites in the fusion protein showing nucleotide substitution compared with LaSota KU665482.1 vaccine strain as reference. Not all substitution resulted in mutation because of degeneracy nature of amino acid. Italic positions show substitution site. Reference strain. †Vaccine strain. ‡Study isolates. HR: heptad repeats; A: adenine; G: guanine; C: cytosine; T: thymine.
