Development and Optimization of a Multiplex Real-Time RT-PCR to Detect SARS-CoV-2 in Human Samples

Figure 2

Amplification curves of different numbers of SARS-CoV-2 genome copies to evaluate the limit of detection (LoD). (a) Detection of 1 viral copy/μL (5 copies per reaction). (b) Detection of 2 viral copies/μL (10 copies per reaction). (c) Detection of 5 viral copies/μL (25 copies per reaction). (d) Detection of 10 viral copies/μL (50 copies per reaction). Reactions were performed in a BioRad CFX96 Thermocycler.

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