International Journal of Surgical Oncology / 2022 / Article / Tab 1 / Research Article
High CD44 Immunoexpression Correlates with Poor Overall Survival: Assessing the Role of Cancer Stem Cell Markers in Oral Squamous Cell Carcinoma Patients from the High-Risk Population of Pakistan Table 1 IHC protocol and antibody details.
S. no. Antibody Clone/product code Source, clonality Company Antibody dilution Antibody incubation Positive control Cellular location 1. CD133 EPR16508 Rabbit, monoclonal Abcam, UK 1 : 1,000 40 min Glioblastoma multiforme Cell membranous and cytoplasmic 2. CD44 DF1485 Mouse, monoclonal Dako, Denmark 1 : 40 40 min Glioblastoma multiforme Cell membranous 3. L1CAM EPR18750 Rabbit, monoclonal Abcam, UK 1 : 500 40 min Normal kidney Cell membranous 4. SOX2 ab97959 Rabbit, polyclonal Abcam, UK 1 : 250 40 min Glioblastoma multiforme Nuclear