Detection of Lactobacillus delbrueckii subsp. bulgaricus and Streptococcus thermophilus in Inactivated Fermented Milk Using Fluorescence Quantitative Loop-Mediated Isothermal Amplification
Table 3
Optimised FQ-LAMP reaction system for L. bulgaricus and S. thermophilus.
Reagent name
Volume (μL)
Reagent sources
L. bulgaricus
S. thermophilus
10× ThermoPol reaction buffer
2.5
2.5
New England Biolabs Inc., USA
MgSO4 (50 mmol/L)
1.0
1.3
8 U Bst DNA polymerase
1.2
1.0
dNTPs (10 mmol/L)
1.5
1.5
Sigma-Aldrich, St. Louis, MO, USA
1/400 dilution 10,000 × SYBR green I
0.3
0.3
Coolaber Science and Technology Co., Ltd., Beijing, China
Betaine (5 mol/L)
2.0
2.0
Leagene Co., Ltd., Beijing, China
F3/B3 (10 μmol/L)
0.5
0.7
Tsingke Biotechnology Co., Ltd., Beijing, China
FIP/BIP (10 μmol/L)
3.5
3.5
Loop (10 μmol/L)
3.5
3.5
DNA template
1.0
1.0
Bacterial DNA extraction kit (Tiangen Biotech Co., Ltd., Beijing, China)
