Modulation of Arginase-2 mRNA Levels by ω-3 PUFAs and Aspirin in Asthmatic Human Lung Fibroblasts

Figure 3

The effects of RvD1, ASA, cAMP, and DEX on ARG2 mRNA levels in HLF. ARG2 mRNA levels were assessed in NHLF (a and c) and DHLF (b and d). HLF were treated with 1 μM RvD1, 10 mM ASA, 100 μM 8-Br-cAMP, or 2 μg/mL DEX for 24 hours. RNA was then extracted from HLF and analyzed by RT-qPCR. Data were statistically analyzed using randomized block ANOVA, followed by Tukey’s multiple comparisons test. Depicted are the mean relative mRNA for biological replicates performed in triplicate for all experiments, except for the cAMP experiment in DHLF (d), for which biological replicates were performed in triplicate. Stars indicate significant differences determined using Tukey’s multiple comparisons test as follows: and significant difference from control.

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