Study of Drug Target Identification and Associated Molecular Mechanisms for the Therapeutic Activity and Hair Follicle Induction of Two Ashwagandha Extracts Having Differential Withanolide Constitutions

Figure 2

Modulation of antioxidant signaling pathway by ASH-Ext1: (a) HEK293, HeLa, and A549 cells were treated with ASH-Ext1 for 24 h and cell lysates were subjected to western blot analysis to measure expression levels of Nrf2, HO1, and GPx1. The bands are representative of three repeat experiments. Target protein expression was normalized to the samples’ loading control GAPDH. Expression levels of (b) Nrf2, (c) HO1, and (d) GPx1 in HEK293, HeLa, and A549 cells. Quantitative data are presented as mean ± SD (standard deviation) of N of 3, and values are considered statistically significant ().

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