A Natural Organic Compound “Decursin” Has Both Antitumor and Renal Protective Effects: Treatment for Osteosarcoma

Figure 2

Decursin induces apoptosis in osteosarcoma cells. (a, b) Human osteosarcoma cells (143B and MG63) were treated with various doses (0–100 µM) of decursin for 24 and 48 h. Apoptosis was assessed via Annexin V-FITC/propidium iodide double staining using flow cytometry. (c, d) Caspase-3 activity was measured using a Caspase-Glo assay kit. The relative ratios are presented. Caspase-3 activity was measured 24 and 48 h after administration of decursin. (e) Western blot analysis was performed using antibodies against Bcl-2 and Bax. β-Actin was used as the loading control. In (a–d), data are expressed as mean ± standard deviation (n = 4).  < 0.05 and  < 0.01, as control. N.S.: not significant.

(a)

(b)

(c)

(d)

(e)