A Natural Organic Compound “Decursin” Has Both Antitumor and Renal Protective Effects: Treatment for Osteosarcoma

Figure 5

Synergy effects of decursin and cisplatin. (a) The 143B and MG63 cells were treated with 0 or 50 μM of decursin and 0 or 5 μM cisplatin for 48 h. The cell viability was assessed using the RealTime-Glo MT cell Viability assay (n = 4). (b) 143B and MG63 cells were administered decursin and/or cisplatin, after which flow cytometry was performed and the percentage of apoptotic cells was calculated at 48 h. Apoptosis was assessed via Annexin V-FITC/propidium iodide double staining, using flow cytometry. (c) Caspase-3 activity was measured using a Caspase-Glo assay kit. The relative ratios are presented. Caspase-3 activity was measured after 48 h of decursin and/or cisplatin treatment. (d) Western blot analysis was performed using various antibodies, including the same ones as those used in Figure 2, after decursin and/or cisplatin treatment. In (a, c), data are expressed as mean ± standard deviation (n = 4).  < 0.05 and  < 0.01. N.S.: not significant.

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