miR-21 Targets ASPP2 to Inhibit Apoptosis via CHOP-Mediated Signaling in Helicobacter pylori-Infected Gastric Cancer Cells

Figure 1

miR-21 is significantly overexpressed in HPGC. (a) Schematic description of the strategy to identify potentially upregulated miRNAs in HPGC. An integrated analysis of both GEO dataset (accession number: GSE62503) and TCGA datasets (data ID: TCGA.STAD.sampleMap/miRNA_HiSeq_gene) was performed, which resulted in the identification of 80 miRNAs that were commonly dysregulated. (b) Volcano plot showed miR-21 as a potentially upregulated miRNA in HPGC among 80 commonly dysregulated miRNA. (c) Relative miR-21 expression levels in HPGC tumor tissues (n = 35) as compared to adjacent normal tissues (n = 35) by in situ hybridization. Upper, representative images were captured at 200x magnification. Scale bar, 50 μm. Lower, relative quantitation of miR-21 levels by H score ranking. The significance of differences in H scores between two groups was compared using the nonparametric Mann–Whitney U test.  < 0.01.

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