miR-21 Targets ASPP2 to Inhibit Apoptosis via CHOP-Mediated Signaling in Helicobacter pylori-Infected Gastric Cancer Cells

Figure 2

Quantitative proteomics reveals altered functional proteome in the GC cells after miR-21 overexpression. (a) Strategic flowchart for the identification of potential miR-21 targets. SILAC-labeled adenovirus-based mock and miR-21-overexpressing AGS cells (AdMock and AdmiR-21) were harvested. Proteins were extracted and digested with trypsin. Peptide mixture was fractionated via offline high-performance liquid chromatography and subsequently analyzed with an LTQ-Orbitrap Velos hybrid mass spectrometer. A total of 47 commonly downregulated proteins (AdmiR-21/AdMock ratio <0.7) were further extracted compared with predicted candidates in the miRsystem. Functional characterization of the 47 leads was performed via IPA to identify the potential miR-21 targets. (b) The eight functional groups that significantly enriched value <0.05) in the 47 candidates of miR-21 targets, where cell death and survival, cellular movement, and cellular growth and proliferation were the top three enriched functions. (c) IPA interaction network analysis shows interacting linkage and functional clustering of molecules among the top three enriched functions. A central molecular hub across all three clusters was formed by the five candidates of miR-21 targets.

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