miR-21 Targets ASPP2 to Inhibit Apoptosis via CHOP-Mediated Signaling in Helicobacter pylori-Infected Gastric Cancer Cells

Figure 6

H. pylori-induced ASPP2 suppression promotes p300-mediated CHOP degradation and inhibits CHOP-mediated apoptotic signaling in the GC cells. (a) AGS and MKN45 cells were incubated with H. pylori at 30 and 90 MOI for the indicated time. Cell lysates were analyzed by immunoblotting using the indicated antibodies. (b) Cell lysates of AGS and MKN45 cells incubated with H. pylori at 30 and 90 MOI for the indicated time were immunoprecipitated with antibodies against ASPP2 (upper) immobilized on protein G beads for 16 hours. (c) Cell lysates of AGS and MKN45 cells incubated with H. pylori at 30 and 90 MOI for the indicated time followed by 10 μM MG132 for 3 h were immunoprecipitated with antibodies against p300 (upper) or CHOP (middle) immobilized on protein A beads for 16 hours. In panels (b) and (c), the Co-IP complex and 20% input (lower) samples were subjected to immunoblotting. All the data were presented as mean ± SD of 3 replicates.  < 0.05,  < 0.01, and  < 0.01.

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