ARV-825 Showed Antitumor Activity against BRD4-NUT Fusion Protein by Targeting the BRD4

Figure 1

Overexpression of BRD4-NUT promotes cell proliferation and migration. (a) CMV-BRD4-NUT vector construction scheme. (b) 48 h after transfection of 3T3 cells with BRD4-NUT vectors, western blot analysis was conducted. (c) Bright field of 3T3 cells, 3T3-BRD4-NUT cells. Scale bars, 200 μm. Cells were further cultured in a complete medium for indicated time periods. (d) Cell migration (wound healing assays). (e) Cell proliferation (CCK8 OD 450 nm) was tested. Data were presented as the mean ± standard deviation (SD, n = 3) (same for all Figures). The experiments were repeated three times, with similar results obtained.

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