ARV-825 Showed Antitumor Activity against BRD4-NUT Fusion Protein by Targeting the BRD4

Figure 2

ARV-825 suppresses the proliferation and migration of BRD4-NUT 3T3 cells. (a) Different concentrations of ARV-825 had little effect on the proliferation of 3T3 cells. (b) Cells overexpressing BRD4-NUT decreased cell proliferation after being treated with ARV-825 at different concentrations in 3T3 cells. (c) 3T3 cell and (d) 3T3-BRD4-NUT cell migration changes after treatment with different concentrations of ARV-825 at specific times. Scale bars, 200 μm. (e) Cells overexpressing BRD4-NUT decreased cell wound recovery after being treated with ARV-825 in 3T3 cells. (f) Western blotting analysis showing the BRD4 protein level in 3T3-BRD4-NUT cells after being treated with ARV-825 at different concentrations for 72 h. (g) 3T3 cells and (h) BRD4-NUT 3T3 cells were treated with 0.03 μm ARV-825 for different times, and the changes of BRD4 protein expression were detected with western blotting.

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