ARV-825 Showed Antitumor Activity against BRD4-NUT Fusion Protein by Targeting the BRD4

Figure 3

Transcriptome difference revealed dynamic changes with ARV-825 treatment. (a) PCA analysis of four sets of data. A projection of the input data set onto the first two principal components. BN, 3T3-BRD4-NUT cells, BN + 0.003, 0.003 μM ARV-825 treated with 3T3-BRD4-NUT cells, BN + 0.03, 0.03 μM ARV-825 treated with 3T3-BRD4-NUT cells. (b) Volcano plot of RNA-seq analysis of gene expression changes in BRD4-NUT 3T3 and control cells. Genes highlighted in blue form the up-fold changes, and cyan indicates down regulated genes and black on behalf of unchanging genes. (c) Heatmap view of the differentially expressed genes in 3T3-BRD4-NUT cells compared with 3T3 cells. Red indicates upregulation, and blue indicates downregulation. (d) KEGG pathway enrichment analysis significantly upregulated after BRD4-NUT overexpression compared with 3T3 cells (top 10). The color represents significance, and the circle size represents the number of genes enriched. (e) The top differentially expressed genes that before and after ARV-825 treatment were subjected to functional enrichment analysis cells ( < 0.05, |log2FC| > 1). Each column represents a different sample, and each row represents a single gene. Color changes within a row indicate expression levels relative to the average of the same population. Red indicates upregulation, blue indicates downregulation, and white indicates the basic level of expression. (f) q-PCR analysis relative gene expression levels of Sox6, Traf1, Cnn1, and Eid3 in each group. Data were presented as the mean ± SD. (n = 4).

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