Journal of Ophthalmology / 2024 / Article / Tab 5 / Review Article
Chemical, Physical, and Biological Corneal Decellularization Methods: A Review of Literature Table 5 The evaluation of the efficiency of physical decellularization methods on corneal tissue and assays used for the evaluation.
Physical decellularization methods Efficiency Assays used for determination of efficiency of corneal decellularization Sources Freeze-thaw cycles +++/++++ Histological analysis with H&E and DAPI staining [27 , 59 ] Hypertonic and hypotonic solutions (mostly NaCl) +++/++++ Histological analysis with H&E and DAPI staining, gel electrophoresis, quantification of DNA [33 , 36 , 37 , 39 , 42 –44 , 47 , 60 , 61 ] High or ultra-high hydrostatic pressure ++++ Histological analysis with H&E staining, quantification of DNA [62 , 63 ] ScCO2 ++++ Histological analysis with H & E and DAPI staining, DNA quantificiation [44 , 64 –66 ]
Note . For evaluating efficiency, we used the “+” symbol, where + is the least efficient decellularization method and +++++ is the most efficient decellularization method.