Research Article

Controlled Release of Mesenchymal Stem Cell-Conditioned Media from a Microsphere/Gel-Based Drug Delivery System for Wound Healing of Tympanic Membrane Perforations

Figure 4

Scratch wound assay images. Representative digital images of scratch wounds in human primary dermal keratinocytes cultured in 24-well plates at time 0, 6, 12, and 24 h. Scratch wounds were incubated in dermal basal media, CM, releasates from blank MS, and releasates from CM-MS. Images were analyzed for scratch wound area using ImageJ. Scale bar = 100 µm. CM, conditioned media; CM-MSs, CM-loaded PLGA microspheres.