Genetically Engineered Macrophages Derived from iPSCs for Self-Regulating Delivery of Anti-Inflammatory Biologic Drugs

Figure 2

(a) Overview of the differentiation protocol for iMAC differentiation from miPSC using stepwise daily feeding with various growth factors and media conditions. (b) Representative images at each stage of differentiation throughout the protocol. (c) qPCR throughout differentiation, normalized to reference gene GAPDH expression at day 0; different letters denote by one-way ANOVA over time in 4-5 independent experiments. (d) Representative flow cytometry of two independent experiments for both early and late markers of primitive hematopoiesis and (e) quantified for mean fluorescence intensity, demonstrated varying but similar increase in marker expression. (f) Representative images of immunocytochemistry of day 17 iMACs for macrophage markers CD11b/CD45 and CD14/CD34 (n = 6) and quantified by flow cytometry (n = 2). Bars represent mean ± SEM.