To assess if (1→3)-β-D-glucan, a microbial cell wall agent normally present in pollen, has the ability to
produce pollenlike response, sensitive persons received a nasal deposition of two doses of (1→3)-β-D-glucan. The percentage of eosinophils and amount of eotaxin were measured
in nasal lavage 30 minutes and 24 hours after challenge. No effect could be demonstrated. The absence of an inflammatory response after (1→3)-β-D-glucan application confirms earlier findings in inhalation studies.