4-Isopropyl-2,6-bis(1-phenylethyl)aniline 1, an Analogue of KTH-13 Isolated from Cordyceps bassiana, Inhibits the NF-κB-Mediated Inflammatory Response

Figure 1

Effects of KTH-13-AD1 on macrophage-mediated inflammatory responses. (a) Chemical structure of KTH-13-AD1. (b) NO production in LPS-treated RAW264.7 cells or induced by sodium nitroprusside (SNP), as determined by Griess assay. ((c), left panel) The ROS scavenging effect of KTH-13-AD1 was measured with SNP (0.25 mM)-treated RAW264.7 cells, using DHR123 (20 μM). The level of ROS was determined through flow cytometric analysis. ((c), right panel) The radical scavenging activity of KTH-13-AD1 was determined using DPPH (250 μM) assay. (d) The effect of KTH-13-AD1 on the viability of RAW264.7 cells was evaluated using MTT assay. and compared to the vehicle control or normal group.

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