Gene Expression Profiling of IL-17A-Treated Synovial Fibroblasts from the Human Temporomandibular Joint

Table 1

Primers used for PCR analysis of genes.

Gene	Primers	Amplicon size (bp)
IL-6	F: 5′-AGC AAA GAG GCA CTG GCA GAA-3′	331
	R: 5′-TTG TCA TGT CCT GCA GCC ACT-3′
CCL20 (MIP-3α)	F: 5′-GCA AGC AAC TTT GAC TGC TG-3′	342
	R: 5′-CAA GTC CAG TGA GGC ACA AA-3′
CXCL1 (GRO-α)	F: 5′-TGC AGG GAA TTC ACC CCA AG-3′	229
	R: 5′-CAG GGC CTC CTT CAG GAA CA-3′
IL-8 (CXCL8)	F: 5′-ACT CCA AAC CTT TCC ACC CCA-3′	129
	R: 5′-TTT CCT TGG GGT CCA GAC AGA-3′
IL-17RA	F: 5′-TTC ATT CCT ATG CCT GAG TC-3′	204
	R: 5′-TAC AGT AAG TGG CTC GAC CT-3′
IL-17RB	F: 5′-CCT CCG AGT AGA ACC TGT TA-3′	200
	R: 5′-GTC TGG TCT GAG TCT GGA AG-3′
IL-17RC	F: 5′-GGA CAA ATA CAT CCA CAA GC-3′	192
	R: 5′-GAG TCA TCG GCT GAG TAG AG-3′
IL-17RD	F: 5′-TGT GCC TTA GAG CAG GTG TG-3′	204
	R: 5′-TGT GCT TGG AAG GGA AAG TC-3′
IL-17RE	F: 5′-GGG TCT CTC ACA TCC TGG AA-3′	207
	R: 5′-CCT CAG GAA GGG AAT GAT GA-3′
GAPDH	F: 5′-ATC ACC ATC TTC CAG GAG-3′	318
	R: 5′-ATG GAC TGT GGT CAT GAG-3′

IL-6, interleukin-6; CCL20, chemokine (CC motif) ligand 20; CXCL1, chemokine (CXC motif) ligand 1; IL-8, interleukin-8; IL-17R (A–E), interleukin-17 receptor (A–E); GAPDH, glyceraldehyde-3-phosphate dehydrogenase; F, forward primer; R, reverse primer.